Stable cell line construction Service

Introduction

Stable cell lines have a wide range of applications in recombinant protein and antibody production, assay analysis, gene editing, functional research and other fields. Tianzhengyuan Bio's stable cell line development service can effectively increase the production capacity of stable cell lines, which can be produced for antibody expression, Fc fusion proteins, a variety of cell surface receptors, cytoplasmic and secreted proteins to improve experimental results. We integrate all experimental steps and provide one-stop technical service from gene sequencing to delivery of stable lines. We offer related services including:

· Gene design and codon optimization

· Multiple expression vector construction

· Transient Transfection and Detection

· Stabilized cell line construction and assay

· Expressive in bulk

Service Description(HEK293 stable cell line development):

Service Steps	Description	Period	Deliverables	Quotation
Gene Synthesis	• Gene Synthesis and Codon Optimization	1week	• Constructed cloning vector • Sequencing Report	info@assayvector.com
Vector construction	• Cloning to expression vectors • Plasmid sequencing • Bulk Plasmid Preparation	1week	• Constructed cloning vector • Sequencing Report
Transient expression assessment	• Small volume transfection of appropriate cells • SDS-PAGE and ELISA analysis to assess protein expression or WB detection of protein expression levels	1week	• Assessment results of Expression
Constructing stable cell lines	• Stable transfection and screening • Identification (qPCR and/or FACS) • Subclone of a cell line • Identification (qPCR and/or FACS) • Stabilized Cell Line Banking	10weeks	• 2-3 stable cell lines • Project Reports
Bulk expression and purification (optional)	• Choice of 1L expanded culture or bioreactor • Affinity Purification • SDS-PAGE Analysis	2-3weeks	• Purified Protein • SDS-PAGE Report • Lab Report

Service Description(CHO stable cell line development)

Service Steps	Description	Period	Deliverables	Quotation
Gene Synthesis	• Gene Synthesis and Codon Optimization	1week	• Constructed cloning vector • Sequencing Report	info@assayvector.com
Vector construction	• Cloning to expression vectors • Plasmid sequencing • Bulk Plasmid Preparation	1week	• Constructed cloning vector • Sequencing Report
Transient expression assessment	• Small volume transfection of appropriate cells • SDS-PAGE and ELISA analysis to assess protein expression or WB detection of protein expression levels	2weeks	• Assessment results of Expression
Constructing stable cell lines	• Stable transfection and screening • High Expression Clone Screening and Stability Screening • Stabilized Cell Line Banking	2-3weeks	• 2-3 stable cell lines • Project Reports
Bulk expression and purification (optional)	• Choice of 1L expanded culture or bioreactor • Affinity Purification • SDS-PAGE Analysis	2-3weeks	• Purified Protein • SDS-PAGE Report • Lab Report